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KMID : 0358119890150020083
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Journal of the Korean Public Health Association
1989 Volume.15 No. 2 p.83 ~ p.90
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HYGIENIC STUDIES ON SOME KOREAN CEREALS
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Eo Y.-J.
Pestka, James, J.
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Abstract
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Rice samples (88 samples harvested in 1987) collected from the whole country except JejuDo were pretreated and aflatoxin B, and zearalenone were analyzed by competitive direct enzyme-linked immunosorbent assay (ELISA) refered to Pestka´s method. Antibody specific for aflatoxin B, produced against afltoxin B, oxime-bovine serum albumin (BSA) conjugates in rabbits had a cross reactivity with afltoxin B2, on the other side, antibody against zearalenone oxime-BSA was cross-reacted with a-zearalenol. Essential features of the procedure were blending rice in methanol-phosphate buffered saline-dimethylformamide with a volume ratio of 70:29: 1, and filtering of the blended sample, and analysis of filtrate by ELISA for aflatoxin B, and zearalenone. Three samples (R-19, R-54, R-64) of 88 rice samples had a positive reaction to aflatoxin Bi, and showed 6.181ig/kg, 2g o2pg/kg and 4. lopg/kg of of latoxin BL level respectively. Meanwhile 9 samples were contaminated to zearalenone, and among them, R-36, R-60 showed high level of zearalenone as 52.12,ug/kg and 14.18,ug/kg.
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KEYWORD
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